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In Reply: In response to Dr Buhimschi and colleagues, we would like to clarify several points. As we noted in the article, our SELDI-TOF proteomic profiling was a low-resolution approach to distinguish patients with and without IAI. The SELDI-TOF mass spectrometry we used (pBluescript II) did not have the necessary mass accuracy to identify potential proteins. The 3- to 5-kDa cluster we identified in IAI contained several peaks and each peak could represent multiple proteins, including defensins. Heine et al¹ described defensins in vaginal secretions by using enzyme-linked immunosorbent assay.

We used well-defined identification methods, de novo sequencing of peptides, and detection of multiple peptides for each protein with appropriate scores, as previously published.² We used 1-dimensional gel separation and in-gel digestion of specific bands for analysis of amniotic fluid proteins in IAI. As stated in the study, we observed peptides derived from IGFBP-1 and the calgranulins within the . . . [Full Text of this Article]

Michael G. Gravett, MD
gravettm@ohsu.edu
Department of Obstetrics & Gynecology

Srinivasa R. Nagalla, MD
Department of Pediatrics
Oregon Health & Science University
ProteoGenix Inc
Portland

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